Anticorps Recombinant de lapin anti-Tyrosinase

Tyrosinase Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242174D2

N° de cat : 84723-3-RR

Synonymes

TYR, 242174D2, EC:1.14.18.1, LB24 AB, LB24-AB

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Galerie de données de validation

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  WB analysis using 84723-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84723-3-RR (Tyrosinase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84723-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84723-3-RR against Human Tyrosinase were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A375, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84723-3-RR cible Tyrosinase dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Tyrosinase Protéine recombinante Ag34940
• Nom complet: tyrosinase (oculocutaneous albinism IA)
• Masse moléculaire calculée: 529 aa, 60 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC027179
• Symbole du gène: TYR
• Identification du gène (NCBI): 7299
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosinase is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. It's a melanocyte-specific enzyme critical for the synthesis of melanin, a process normally restricted to a post-Golgi compartment termed the melanosome. Loss-of-function mutations in tyrosinase are the cause of oculocutaneous albinism. The 60-kDa tyrosinase core polypeptide is modified in the endoplasmic reticulum (ER) by cotranslational addition of multiple N-linked glycans, producing the 70-kDa species. Complex sugar modifications in the Golgi apparatus further increases tyrosinase's molecular mass to 80 kDa, the size of the mature wild-type (WT) isoform. (PMID:10823941)

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