Anticorps Polyclonal de lapin anti-U2AF2/U2AF65

• Phare
• Validé par KD/KO

U2AF2/U2AF65 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, RIP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15624-1-AP

Synonymes

hU2AF(65), hU2AF65, U2AF2, U2AF65

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Galerie de données de validation

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  WB analysis of HepG2 using 15624-1-AP

  WB result of U2AF65 antibody (15624-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF65 transfected HepG2 cells.

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  WB analysis of Jurkat using 15624-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 15624-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15624-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15624-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15624-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293T, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

15624-1-AP cible U2AF2/U2AF65 dans les applications de WB, RIP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: U2AF2/U2AF65 Protéine recombinante Ag8070
• Nom complet: U2 small nuclear RNA auxiliary factor 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC008740
• Symbole du gène: U2AF2
• Identification du gène (NCBI): 11338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	IF,RIP	J Clin Invest; Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.; Authors - Xue Gong
human	WB	Sci Rep; Identification of novel PANDAR protein interaction partners involved in splicing regulation.; Authors - N Pospiech
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human	WB	Cell Death Dis; RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.; Authors - Shicheng Sun
human	WB	J Immunol Res; Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.; Authors - Li Fang