Anticorps Monoclonal anti-UAP1

• Phare
• Validé par KD/KO

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2C7B2

N° de cat : 67545-1-Ig

Synonymes

2C7B2, AgX, AGX 2, AGX1, Antigen X

---

Galerie de données de validation

• 

  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67545-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67545-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 67545-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules DU 145, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NCI-H1299, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67545-1-Ig cible UAP1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UAP1 Protéine recombinante Ag9513
• Nom complet: UDP-N-acteylglucosamine pyrophosphorylase 1
• Masse moléculaire calculée: 505 aa, 57 kDa
• Poids moléculaire observé: 57-59 kDa
• Numéro d’acquisition GenBank: BC009377
• Symbole du gène: UAP1
• Identification du gène (NCBI): 6675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

Publications

Species	Application	Title
human	WB,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	WB	Mol Cell; Metabolic enzyme UAP1 mediates IRF3 pyrophosphorylation to facilitate innate immune response; Authors - Shuai Yang ; KD Validated
human	WB,IHC	Front Immunol; The hexosamine biosynthesis pathway-related gene signature correlates with immune infiltration and predicts prognosis of patients with osteosarcoma; Authors - Zexin Su

{{ptg:RelatedPrimaryAntibodies}}