Anticorps Monoclonal anti-UAP1

• Phare
• Validé par KD/KO

UAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C7B2

N° de cat : 67545-1-PBS

Synonymes

2C7B2, AgX, AGX 2, AGX1, Antigen X

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Galerie de données de validation

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  WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  WB analysis using 67545-1-Ig (same clone as 67545-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67545-1-Ig (UAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67545-1-Ig (UAP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 67545-1-Ig (same clone as 67545-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using UAP1 antibody (67545-1-Ig, Clone: 2C7B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67545-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67545-1-PBS cible UAP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UAP1 Protéine recombinante Ag9513
• Nom complet: UDP-N-acteylglucosamine pyrophosphorylase 1
• Masse moléculaire calculée: 505 aa, 57 kDa
• Poids moléculaire observé: 57-59 kDa
• Numéro d’acquisition GenBank: BC009377
• Symbole du gène: UAP1
• Identification du gène (NCBI): 6675
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UAP1 can convert UTP and GlcNAc-1-P into UDP-GlcNAc, it can also convert UTP and GalNAc-1-P into UDP-GalNAc. There are three isoforms of UAP1, among which isoform  AGX1  has 2 to 3 times higher activity towards GalNAc-1-P, while isoform  AGX2  has 8 times more activity towards GlcNAc-1-P. It has been reported that UAP1 is specific and highly expressed in prostate cancer. UAP1 can protect cells against inhibitors of N-linked glycosylation. UAP1 may play an important role in maintaining INS responsiveness. 67545-1-Ig detects 57 and 59 kDa isoforms in SDS-PAGE.(PMID:25241896)

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