Anticorps Monoclonal anti-UBA3

• Phare
• Validé par KD/KO

UBA3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1C10D8

N° de cat : 68067-1-Ig

Synonymes

1C10D8, EC:6.2.1.64, hUBA3, NEDD8 activating enzyme E1C, NEDD8-activating enzyme E1 catalytic subunit

---

Galerie de données de validation

• 

  WB analysis using 68067-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68067-1-Ig (UBA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HEK-293 using 68067-1-Ig

  WB result of UBA3 antibody (68067-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA3 transfected HEK-293 cells.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

68067-1-Ig cible UBA3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBA3 Protéine recombinante Ag31051
• Nom complet: ubiquitin-like modifier activating enzyme 3
• Masse moléculaire calculée: 463 aa, 52 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC022853
• Symbole du gène: UBA3
• Identification du gène (NCBI): 9039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Publications

Species	Application	Title
	WB	Funct Integr Genomics; Upregulation of Cullin1 neddylation promotes glycolysis and M1 polarization of macrophage via NF-κB p65 pathway in sepsis; Authors - Fuchuang Qin

{{ptg:RelatedPrimaryAntibodies}}