Anticorps Monoclonal anti-UBA3

• Phare
• Validé par KD/KO

UBA3 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C10D8

N° de cat : 68067-1-PBS

Synonymes

1C10D8, EC:6.2.1.64, hUBA3, NEDD8 activating enzyme E1C, NEDD8-activating enzyme E1 catalytic subunit

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Galerie de données de validation

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  WB analysis using 68067-1-Ig (same clone as 68067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68067-1-Ig (UBA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68067-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68067-1-Ig (same clone as 68067-1-PBS)

  WB result of UBA3 antibody (68067-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA3 transfected HEK-293 cells. This data was developed using the same antibody clone with 68067-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68067-1-PBS cible UBA3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBA3 Protéine recombinante Ag31051
• Nom complet: ubiquitin-like modifier activating enzyme 3
• Masse moléculaire calculée: 463 aa, 52 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC022853
• Symbole du gène: UBA3
• Identification du gène (NCBI): 9039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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