Anticorps Polyclonal de lapin anti-UBASH3A-Specific

UBASH3A-Specific Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15823-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15823-1-AP (same clone as 15823-1-PBS)

  mouse bone marrow tissue were subjected to SDS PAGE followed by western blot with 15823-1-AP (UBASH3A-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse spleen using 15823-1-AP (same clone as 15823-1-PBS)

  IP result of anti-UBASH3A-Specific (IP:15823-1-AP, 4ug; Detection:15823-1-AP 1:500) with mouse spleen tissue lysate 1920 ug. This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 15823-1-AP (same clone as 15823-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using UBASH3A-Specific antibody (15823-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15823-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15823-1-PBS cible UBASH3A-Specific dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ubiquitin associated and SH3 domain containing, A
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 70-74 kDa
• Numéro d’acquisition GenBank: NM_018961
• Symbole du gène: UBASH3A
• Identification du gène (NCBI): 53347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBASH3A gene encodes ubiquitin-associated and SH3 domain-containing protein A (UBASH3A) also known as T-cell ubiquitin ligand 1 (TULA1) which is one of two family members belonging to the T-cell ubiquitin ligand (TULA) family. UBASH3A gene expression is limited to only a few tissues, with its highest expression in spleen, peripheral blood leukocytes, and bone marrow. UBASH3A protein is associated with c-Cbl and ubiquitylated proteins and has been implicated in the regulation of signaling mediated by protein-tyrosine kinases oth family members can negatively regulate T-cell signaling. UBASH3A can facilitate growth factor withdrawal-induced apoptosis in T cells via its interaction with apoptosis-inducing factor (AIF). This antibody is UBASH3A-specific.

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