Anticorps Polyclonal de lapin anti-UBASH3B/STS 1

• Phare
• Validé par KD/KO

UBASH3B/STS 1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 19563-1-AP

Synonymes

Cbl interacting protein p70, KIAA1959, p70, STS 1, STS1, UBASH3B, UBASH3B/STS 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 19563-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19563-1-AP

  WB result of UBASH3B/STS 1 antibody (19563-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBASH3B/STS 1 transfected HEK-293 cells.

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  WB analysis of mouse lung using 19563-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 19563-1-AP

  IP Result of anti-UBASH3B/STS 1 (IP:19563-1-AP, 3ug; Detection:19563-1-AP 1:300) with mouse kidney tissue lysate 6000ug.

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19563-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19563-1-AP (UBASH3B/STS 1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu pulmonaire de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

19563-1-AP cible UBASH3B/STS 1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ubiquitin associated and SH3 domain containing, B
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: NM_032873
• Symbole du gène: UBASH3B
• Identification du gène (NCBI): 84959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBASH3B, also named STS1 or TULA-2, is a protein that contains a ubiquitin-associated domain at the N-terminus, a SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. TULA-2 is supposed to form homodimer and interact with ubiquitin and mono-ubiquitinated proteins. TULA-2 functions to promote accumulation of activated target receptors, such as T-cell receptors and EGFR on the cell surface.

Publications

Species	Application	Title
human	WB	Cell; Oncogenic Mutations Rewire Signaling Pathways by Switching Protein Recruitment to Phosphotyrosine Sites.; Authors - Alicia Lundby
human,mouse	WB	Biochimie; Ubash3b promotes TPA-mediated suppression of leukemogenesis through accelerated downregulation of PKCδ protein.; Authors - Yao Yao ; KD Validated
mouse	WB	Front Immunol; Targeting the Cbl-b-Notch1 axis as a novel immunotherapeutic strategy to boost CD8+ T-cell responses; Authors - Giulia Monticone ; KO Validated
human	WB	Int J Mol Sci; STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression; Authors - Na Li
human	WB	Transl Cancer Res; Prazosin inhibits the growth and mobility of osteosarcoma cells; Authors - Ming An
human,mouse	WB,IF	Cell Rep; PSGL-1 attenuates early TCR signaling to suppress CD8+ T cell progenitor differentiation and elicit terminal CD8+ T cell exhaustion; Authors - Jennifer L Hope