Anticorps Polyclonal de lapin anti-UBC12

• Phare
• Validé par KD/KO

UBC12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 14520-1-AP

Synonymes

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M

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Galerie de données de validation

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  WB analysis using 14520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14520-1-AP

  WB result of UBC12 antibody (14520-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells.

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  WB analysis of L02 using 14520-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 14520-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP (UBC12 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of L02 using 14520-1-AP

  IP Result of anti-UBC12 (IP:14520-1-AP, 4ug; Detection:14520-1-AP 1:600) with L02 cells lysate 1800ug.

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  IHC staining of mouse ovary using 14520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 14520-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14520-1-AP (UBC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of L02 using 14520-1-AP

  Immunofluorescent analysis of L02 cells using 14520-1-AP (UBC12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules L02, cellules Ramos, cellules SMMC-7721
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu ovarien de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14520-1-AP cible UBC12 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBC12 Protéine recombinante Ag5984
• Nom complet: ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC058924
• Symbole du gène: UBE2M
• Identification du gène (NCBI): 9040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.; Authors - Haibin Zhou
human	WB	Elife; Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.; Authors - Maria Fomicheva ; KO Validated
human	WB	Cancer Biol Med; CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.; Authors - Ting Li
human	IHC,WB, IF	Exp Cell Res; NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.; Authors - Xin Zhao
human	WB	Biochem Biophys Res Commun; Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.; Authors - Xiaoling Hong
human	WB	Biochem Cell Biol; Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.; Authors - Wenjuan Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Maria (Verified Customer) (02-06-2018)

I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250-1:500
• Cell Tissue Type: EpH4 mammary gland cell line

UBC12 Antibody Western Blot, validation (1:250-1:500 dilution) in EpH4 mammary gland cell line (Cat no:14520-1-AP)