Anticorps Monoclonal anti-UBC12

• Phare
• Validé par KD/KO

UBC12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1H8D2

N° de cat : 67482-1-PBS

Synonymes

UBE2M, EC:2.3.2.34, hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12

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Galerie de données de validation

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  WB analysis using 67482-1-Ig (same clone as 67482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67482-1-Ig (UBC12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67482-1-Ig (same clone as 67482-1-PBS)

  WB result of UBC12 antibody (67482-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBC12 transfected HEK-293 cells. This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67482-1-Ig (UBC12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67482-1-Ig (same clone as 67482-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBC12 antibody (67482-1-Ig, Clone: 1H8D2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67482-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67482-1-PBS cible UBC12 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBC12 Protéine recombinante Ag5984
• Nom complet: ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC058924
• Symbole du gène: UBC12
• Identification du gène (NCBI): 9040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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