Anticorps Polyclonal de lapin anti-UBC9

UBC9 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10224-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse spleen using 10224-1-AP (same clone as 10224-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10224-1-AP (same clone as 10224-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 10224-1-AP (same clone as 10224-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 10224-1-AP (same clone as 10224-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10224-1-AP (same clone as 10224-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10224-1-AP (same clone as 10224-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10224-1-AP (UBC9 antibody) at dilution of 1:50 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 10224-1-AP (same clone as 10224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10224-1-AP (UBC9 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10224-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10224-1-PBS cible UBC9 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBC9 Protéine recombinante Ag0252
• Nom complet: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC000427
• Symbole du gène: UBC9
• Identification du gène (NCBI): 7329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

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