Anticorps Monoclonal anti-UBC9

UBC9 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

4A10B11

Avis (1)

N° de cat : 60201-1-Ig

Synonymes

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

---

Galerie de données de validation

• 

  WB analysis using 60201-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 60201-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 60201-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60201-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 60201-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug UBC9 antibody (60201-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules PC-12, tissu rénal de rat
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:600
• Flow Cytometry (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60201-1-Ig cible UBC9 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBC9 Protéine recombinante Ag7885
• Nom complet: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC004437
• Symbole du gène: UBE2I
• Identification du gène (NCBI): 7329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Publications

Species	Application	Title
human,monkey	WB,IF	Viruses; The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation; Authors - Congcong Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Eiko (Verified Customer) (12-02-2020)

This UBC9 mouse monoclonal antibody worked very well in my western blotting. For blocking & dilution of antibody, I used 5% skim milk TBS-T.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hTERT-RPE1 cells U-2 OS cells