Anticorps Monoclonal anti-UBC9

UBC9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A10B11

N° de cat : 60201-1-PBS

Synonymes

UBE2I, 4A10B11, C358B7.1, E2,UBE2I, EC:2.3.2.-

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Galerie de données de validation

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  WB analysis using 60201-1-Ig (same clone as 60201-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 60201-1-Ig (same clone as 60201-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 60201-1-Ig (UBC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60201-1-Ig (same clone as 60201-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60201-1-Ig (UBC9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60201-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60201-1-PBS cible UBC9 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBC9 Protéine recombinante Ag7885
• Nom complet: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC004437
• Symbole du gène: UBC9
• Identification du gène (NCBI): 7329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

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