Anticorps Polyclonal de lapin anti-UBE2C

• Phare
• Validé par KD/KO

UBE2C Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 12134-2-AP

Synonymes

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C

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Galerie de données de validation

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  WB analysis of HeLa using 12134-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12134-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12134-2-AP (UBE2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12134-2-AP

  IP Result of anti-UBE2C (IP:12134-2-AP, 3ug; Detection:12134-2-AP 1:300) with HeLa cells lysate 2440ug.

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  IHC staining of human colon cancer using 12134-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12134-2-AP (UBE2C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12134-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12134-2-AP (UBE2C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

12134-2-AP cible UBE2C dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2C Protéine recombinante Ag2780
• Nom complet: ubiquitin-conjugating enzyme E2C
• Masse moléculaire calculée: 179 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC016292
• Symbole du gène: UBE2C
• Identification du gène (NCBI): 11065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation (PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

Publications

Species	Application	Title
	WB	Plant Cell; SINAT E3 Ubiquitin Ligases Mediate FREE1 and VPS23A Degradation to Modulate Abscisic Acid Signaling.; Authors - Fan-Nv Xia
human	WB,IF	Arterioscler Thromb Vasc Biol; Disturbed Flow Increases UBE2C (Ubiquitin E2 Ligase C) via Loss of miR-483-3p, Inducing Aortic Valve Calcification by the pVHL (von Hippel-Lindau Protein) and HIF-1α (Hypoxia-Inducible Factor-1α) Pathway in Endothelial Cells.; Authors - Joan Fernandez Esmerats
human	WB,IHC	Cell Biosci; DNA primase subunit 1 deteriorated progression of hepatocellular carcinoma by activating AKT/mTOR signaling and UBE2C-mediated P53 ubiquitination.; Authors - Mengqi Zhu
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang
mouse	WB，IHC	Front Genet; Identifying the Key Genes in Mouse Liver Regeneration After Partial Hepatectomy by Bioinformatics Analysis and in vitro/vivo Experiments.; Authors - Jian Zhao
human	WB,IHC	Am J Transl Res; RIZ1 negatively regulates ubiquitin-conjugating enzyme E2C/UbcH10 via targeting c-Myc in meningioma.; Authors - Zheng Cai ; KD Validated