Anticorps Polyclonal de lapin anti-UBE2D1/2/3/4

UBE2D1/2/3/4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28328-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28328-1-AP (same clone as 28328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28328-1-AP (same clone as 28328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 28328-1-AP (same clone as 28328-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 28328-1-AP (same clone as 28328-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28328-1-AP (UBE2D2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28328-1-AP (same clone as 28328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28328-1-AP (same clone as 28328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28328-1-AP (UBE2D1/2/3/4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28328-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28328-1-PBS cible UBE2D1/2/3/4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2D1/2/3/4 Protéine recombinante Ag28736
• Nom complet: ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 14-17 kDa
• Numéro d’acquisition GenBank: BC033349
• Symbole du gène: UBE2D2
• Identification du gène (NCBI): 7322
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

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