Anticorps Monoclonal anti-UBE2D1/2/3/4

UBE2D1/2/3/4 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1C7D12

N° de cat : 68138-1-Ig

Synonymes

UBE2D3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, E2 ubiquitin-conjugating enzyme D3, E2(17)KB3, EC:2.3.2.23

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Galerie de données de validation

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  WB analysis using 68138-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68138-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68138-1-Ig (UBE2D3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IF Staining of A431 using 68138-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBE2D1/2/3/4 antibody (68138-1-Ig, Clone: 1C7D12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68138-1-Ig cible UBE2D1/2/3/4 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBE2D1/2/3/4 Protéine recombinante Ag2278
• Nom complet: ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC003395
• Symbole du gène: UBE2D3
• Identification du gène (NCBI): 7323
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

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