Anticorps Polyclonal de lapin anti-UBE2E2

• Phare
• Validé par KD/KO

UBE2E2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11844-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of human thyroid gland using 11844-1-AP (same clone as 11844-1-PBS)

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11844-1-AP (same clone as 11844-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse embryo using 11844-1-AP (same clone as 11844-1-PBS)

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 11844-1-AP (UBE2E2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11844-1-AP (same clone as 11844-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11844-1-AP (UBE2E2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11844-1-AP (same clone as 11844-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using UBE2E2 antibody 11844-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 11844-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11844-1-PBS cible UBE2E2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2E2 Protéine recombinante Ag2509
• Nom complet: ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog, yeast)
• Masse moléculaire calculée: 201 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC022332
• Symbole du gène: UBE2E2
• Identification du gène (NCBI): 7325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBE2E2(ubiquitin-conjugating enzyme E2 E2) is also named as UBCH8 and belongs to the ubiquitin-conjugating enzyme family. This gene encodes a 22 kDa protein that functions as a major ISG15 E2 enzyme raises the possibility that ISG15 conjugation might use UbcH8-competent Ub E3 enzymes and also function as an E2 for several Ub E3 enzymes, including both HECT E3s and RING E3s(PMID: 15131269).

{{ptg:RelatedPrimaryAntibodies}}