Anticorps Polyclonal de lapin anti-UBE2F

UBE2F Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17056-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 17056-1-AP (same clone as 17056-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 17056-1-AP (same clone as 17056-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 17056-1-AP (same clone as 17056-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17056-1-AP (UBE2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 17056-1-AP (same clone as 17056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin using 17056-1-AP (UBE2F antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 17056-1-AP (same clone as 17056-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 17056-1-AP (UBE2F antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17056-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

17056-1-PBS cible UBE2F dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2F Protéine recombinante Ag10764
• Nom complet: ubiquitin-conjugating enzyme E2F (putative)
• Masse moléculaire calculée: 185 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC010549
• Symbole du gène: UBE2F
• Identification du gène (NCBI): 140739
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBE2F(Ubiquitin-conjugating enzyme E2 F) is also named as NCE2(NEDD8-conjugating enzyme 2) and belongs to the ubiquitin-conjugating enzyme family.The canonical uBE2M catalyses the modification of most cullins, whereas uBE2F is specifically responsible for cuL5 neddylation. uBE2F is regulated differently than uBE2M, supporting the idea that neddylation is used to fine-tune ubiquitin-chain formation by different cullin-dependent E3s(PMID:19465892).

{{ptg:RelatedPrimaryAntibodies}}