Anticorps Polyclonal de lapin anti-UBE2H

UBE2H Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 15685-1-AP

Synonymes

UBC8, EC:2.3.2.24, EC:2.3.2.23, E2 ubiquitin-conjugating enzyme H, E2 20K

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Galerie de données de validation

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  WB analysis using 15685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15685-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15685-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15685-1-AP (UBE2H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 15685-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15685-1-AP (UBE2H antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

15685-1-AP cible UBE2H dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2H Protéine recombinante Ag8187
• Nom complet: ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast)
• Masse moléculaire calculée: 183 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC006277
• Symbole du gène: UBE2H
• Identification du gène (NCBI): 7328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth; Authors - Nelson V Simwela
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi
mouse	WB	Nat Commun; Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth; Authors - Nelson V Simwela
human	WB	Cell Rep; mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase; Authors - Brittany Q Pham

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

My attempt to use this antibody to detect UBE2H in HEK293T failed. The band intensity was very low, and there were many non-specific bands as well. I used bis-Tris gel and transferred the proteins to PVDF membrane. Primary antibody was incubated over night at 4'C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

UBE2H Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:15685-1-AP)

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