Anticorps Polyclonal de lapin anti-UBC9-Specific

UBC9-Specific Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 14837-1-AP

Synonymes

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBC9-Specific, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I

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Galerie de données de validation

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  WB analysis using 14837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14837-1-AP (UBC9-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14837-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14837-1-AP (UBC9-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14837-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14837-1-AP (UBC9-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14837-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14837-1-AP (UBC9-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 14837-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug UBC9-Specific antibody (14837-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules U-937, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14837-1-AP cible UBC9-Specific dans les applications de WB, IHC, IF, FC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: NM_003345
• Symbole du gène: UBE2I
• Identification du gène (NCBI): 7329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Praveen (Verified Customer) (03-13-2019)

Excellent

• Applications: Western Blot,
• Cell Tissue Type: AC16

FH

Shubham (Verified Customer) (03-12-2019)

Very good antibody with a prominent band.

• Applications: Western Blot,
• Cell Tissue Type: AC16

UBC9-Specific Antibody Western Blot, validation ( dilution) in AC16 (Cat no:14837-1-AP)