Anticorps Polyclonal de lapin anti-UBE2S

UBE2S Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 18219-1-AP

Synonymes

OK/SW-cl.73, EPF5, EC:2.3.2.23, E2-EPF, E2EPF

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Galerie de données de validation

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  WB analysis of HL-60 using 18219-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18219-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 18219-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18219-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 18219-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18219-1-AP (UBE2S antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 18219-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18219-1-AP (UBE2S antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 18219-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18219-1-AP (UBE2S antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules COLO 320, cellules MCF-7, cellules MDA-MB-453s, tissu ovarien de souris
• Résultats positifs en IHC: tissu de cancer de la peau humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18219-1-AP cible UBE2S dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2S Protéine recombinante Ag12978
• Nom complet: ubiquitin-conjugating enzyme E2S
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC004236
• Symbole du gène: UBE2S
• Identification du gène (NCBI): 27338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

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