Anticorps Recombinant de lapin anti-UBE2S

UBE2S Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6D18

N° de cat : 81854-1-PBS

Synonymes

6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

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Galerie de données de validation

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  WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81854-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81854-1-PBS cible UBE2S dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UBE2S Protéine recombinante Ag5264
• Nom complet: ubiquitin-conjugating enzyme E2S
• Masse moléculaire calculée: 222 aa, 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC066948
• Symbole du gène: UBE2S
• Identification du gène (NCBI): 27338
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

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