Anticorps Polyclonal de lapin anti-UBE2T/HSPC150

• Phare
• Validé par KD/KO

UBE2T/HSPC150 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 10105-2-AP

Synonymes

HSPC150, PIG50, UBE2T, UBE2T/HSPC150, Ubiquitin carrier protein T, Ubiquitin protein ligase T

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Galerie de données de validation

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  WB analysis of HeLa using 10105-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10105-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10105-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10105-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10105-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10105-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10105-2-AP (UBE2T/HSPC150 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10105-2-AP

  IP Result of anti-UBE2T/HSPC150 (IP:10105-2-AP, 3ug; Detection:10105-2-AP 1:500) with HeLa cells lysate 3000ug.

• 

  IF Staining of HepG2 using 10105-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UBE2T/HSPC150 antibody (10105-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules SKOV-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10105-2-AP cible UBE2T/HSPC150 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE2T/HSPC150 Protéine recombinante Ag0153
• Nom complet: ubiquitin-conjugating enzyme E2T (putative)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC004152
• Symbole du gène: UBE2T
• Identification du gène (NCBI): 29089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Publications

Species	Application	Title
human	WB	Am J Hum Genet; Mutations in the Gene Encoding the E2 Conjugating Enzyme UBE2T Cause Fanconi Anemia.; Authors - Asuka Hira
human	WB	Cancer Res; Proteasome function is required for DNA damage response and fanconi anemia pathway activation.; Authors - Céline Jacquemont
human	WB	Blood Adv; Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.; Authors - David A Alagpulinsa
human	WB,IHC	Oncotarget; UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.; Authors - Wei Hu ; KD Validated
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang
		Tumour Biol; Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression.; Authors - Hao Yu