Anticorps Monoclonal anti-UBE3A

UBE3A Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

3D6H9

N° de cat : 60038-1-PBS

Synonymes

3D6H9, ANCR, AS, E6 AP, E6AP

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Galerie de données de validation

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  WB analysis of HeLa using 60038-1-Ig (same clone as 60038-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60038-1-Ig (UBE3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

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  Sample test of MP51408-1

  Sample test of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS.

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  IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60038-1-Ig (same clone as 60038-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60038-1-Ig(UBE3A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60038-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51408-1

  Cytometric bead array standard curve of MP51408-1, UBE3A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60038-1-PBS. Detection antibody: 60977-1-PBS. Standard:Ag17546. Range: 3.125-400 ng/mL .

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Informations sur le produit

60038-1-PBS cible UBE3A dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBE3A Protéine recombinante Ag0346
• Nom complet: ubiquitin protein ligase E3A
• Masse moléculaire calculée: 852 aa, 98 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC002582
• Symbole du gène: UBE3A
• Identification du gène (NCBI): 7337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBE3A(Ubiquitin-protein ligase E3A) is also named as E6AP, EPVE6AP, HPVE6A and belongs to the E3 ubiquitin protein ligase family. It functions as both an E3 ligase in the ubiquitin proteasome pathway and as a transcriptional coactivator. It is also initially identified as a cellular protein that mediates in vitro association of the human papillomavirus E6 protein with p53, leading to the ubiquitin-dependent degradation of p53(PMID:1661671). Defects in UBE3A are a cause of Angelman syndrome (AS)(PMID:10508479 ). It has 3 isoforms produced by alternative splicing.

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