Anticorps Polyclonal de lapin anti-UBE4A

• Phare
• Validé par KD/KO

UBE4A Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21548-1-AP

Synonymes

E4, KIAA0126, UBE4A, UBOX2, UFD2

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Galerie de données de validation

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  WB analysis of HEK-293 using 21548-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21548-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21548-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21548-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21548-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21548-1-AP (UBE4A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21548-1-AP

  IP result of anti-UBE4A (IP:21548-1-AP, 4ug; Detection:21548-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules L02, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

21548-1-AP cible UBE4A dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBE4A Protéine recombinante Ag16211
• Nom complet: ubiquitination factor E4A (UFD2 homolog, yeast)
• Masse moléculaire calculée: 1073 aa, 124 kDa
• Poids moléculaire observé: 123 kDa
• Numéro d’acquisition GenBank: BC112367
• Symbole du gène: UBE4A
• Identification du gène (NCBI): 9354
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu
human	WB	Biochim Biophys Acta Gene Regul Mech; UBE4A catalyzes NRF1 ubiquitination and facilitates DDI2-mediated NRF1 cleavage; Authors - Xianyan Hu ; KD Validated

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