Anticorps Recombinant de lapin anti-UBE4B

• Phare
• Validé par KD/KO

UBE4B Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242218E4

N° de cat : 85332-2-PBS

Synonymes

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

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Galerie de données de validation

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  WB analysis using 85332-2-RR (same clone as 85332-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85332-2-RR (UBE4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

  WB result of UBE4B antibody (85332-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells. This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85332-2-RR (same clone as 85332-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE4B antibody (85332-2-RR, Clone: 242218E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85332-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85332-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85332-2-RR against Human UBE4B were performed. The affinity constant is 0.895 nM.

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Informations sur le produit

85332-2-PBS cible UBE4B dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UBE4B Protéine recombinante Ag12836
• Nom complet: ubiquitination factor E4B (UFD2 homolog, yeast)
• Masse moléculaire calculée: 1173 aa, 133 kDa
• Poids moléculaire observé: 140-146 kDa
• Numéro d’acquisition GenBank: BC093696
• Symbole du gène: UBE4B
• Identification du gène (NCBI): 10277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.

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