Anticorps Recombinant de lapin anti-UBE4B

• Phare
• Validé par KD/KO

UBE4B Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242218E4

N° de cat : 85332-2-RR

Synonymes

EC:2.3.2.27, HDNB1, KIAA0684, UBOX3, UFD2

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Galerie de données de validation

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  WB analysis using 85332-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85332-2-RR (UBE4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85332-2-RR

  WB result of UBE4B antibody (85332-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE4B transfected HeLa cells.

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  IF Staining of HeLa using 85332-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE4B antibody (85332-2-RR, Clone: 242218E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85332-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85332-2-RR against Human UBE4B were performed. The affinity constant is 0.895 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules HepG2, cellules K-562, cellules Raji, cellules RAW 264.7, tissu testiculaire de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85332-2-RR cible UBE4B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UBE4B Protéine recombinante Ag12836
• Nom complet: ubiquitination factor E4B (UFD2 homolog, yeast)
• Masse moléculaire calculée: 1173 aa, 133 kDa
• Poids moléculaire observé: 140-146 kDa
• Numéro d’acquisition GenBank: BC093696
• Symbole du gène: UBE4B
• Identification du gène (NCBI): 10277
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBE4B (Ubiquitin Conjugation E4 B) is a protein-coding gene that plays a crucial role in the ubiquitination process, which is essential for protein degradation and regulation of various cellular processes. UBE4B functions as both an E3 and E4 ubiquitin ligase. It collaborates with other ubiquitin ligases to catalyze the transfer of ubiquitin molecules to target proteins, leading to their degradation or other intracellular sorting events. For example, UBE4B has been shown to ubiquitinate and degrade proteins such as Ku70, c-FLIPL, and p53. It also interacts with the E3 ubiquitin ligase ITCH to induce polyubiquitination and proteasomal degradation of target proteins.

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