Anticorps Polyclonal de lapin anti-BMSC UbP

BMSC UbP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 11564-1-AP

Synonymes

BMSC UbP, BMSCUBP, TCBA1, Ubiquitin like protein 7, Ubiquitin like protein SB132, UBL7

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 11564-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11564-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11564-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11564-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11564-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11564-1-AP (BMSC UbP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11564-1-AP

  IP Result of anti-BMSC UbP (IP:11564-1-AP, 4ug; Detection:11564-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human gliomas using 11564-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11564-1-AP (BMSC UbP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 11564-1-AP

  Immunofluorescent analysis of HepG2 cells using 11564-1-AP (BMSC UbP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa, cellules HepG2, cellules Jurkat, tissu testiculaire de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11564-1-AP cible BMSC UbP dans les applications de WB, IP, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BMSC UbP Protéine recombinante Ag2130
• Nom complet: ubiquitin-like 7 (bone marrow stromal cell-derived)
• Masse moléculaire calculée: 380 aa, 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC030055
• Symbole du gène: UBL7
• Identification du gène (NCBI): 84993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.