Anticorps Polyclonal de lapin anti-UBQLN2

UBQLN2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 23449-1-AP

Synonymes

CHAP1, CHAP1/DSK2, Dsk2, DSK2 homolog, hPLIC 2, HRIHFB2157, LIC 2, N4BP4, PLIC 2, PLIC2, RIHFB2157, ubiquilin 2, Ubiquilin-2, UBQLN2

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Galerie de données de validation

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  WB analysis of rat brain using 23449-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23449-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23449-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23449-1-AP (UBQLN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23449-1-AP (UBQLN2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 23449-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 23449-1-AP (UBQLN2 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 23449-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN2 antibody (23449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HEK-293, cellules HepG2
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

23449-1-AP cible UBQLN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBQLN2 Protéine recombinante Ag20149
• Nom complet: ubiquilin 2
• Masse moléculaire calculée: 624 aa, 66 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC069237
• Symbole du gène: UBQLN2
• Identification du gène (NCBI): 29978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Brain; Tamoxifen improves muscle structure and function of Bin1- and Dnm2-related centronuclear myopathies; Authors - Charlotte Gineste
		Hum Mol Genet; Conditional depletion of intellectual disability and Parkinsonism candidate gene ATP6AP2 in fly and mouse induces cognitive impairment and neurodegeneration.; Authors - Aline Dubos
mouse	IF	Brain Pathol; Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD.; Authors - Nicole R Higgins
	WB	J Neurochem; Pathogenic mutation of UBQLN2 impairs its interaction with UBXD8 and disrupts endoplasmic reticulum-associated protein degradation.; Authors - Yuxing Xia
human	WB,IF,IP	F1000Res; The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence; Authors - Ian McDowell ; KO Validated
	WB	bioRxiv; Short N-terminal disordered regions and the proline-rich domain are major regulators of phase transitions for full-length UBQLN1, UBQLN2 and UBQLN4; Authors - Thuy P Dao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (06-22-2023)

Antibody quality was good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

UBQLN2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:23449-1-AP)