Anticorps Monoclonal anti-UBR5

• Phare
• Validé par KD/KO

UBR5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7A6

N° de cat : 66937-1-PBS

Synonymes

DD5, EDD, EDD1, FLJ11310, hHYD, HYD, KIAA0896, Progestin induced protein, UBR5

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Galerie de données de validation

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  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  WB result of UBR5 antibody (66937-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBR5 transfected HeLa cells. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66937-1-Ig (same clone as 66937-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66937-1-Ig (same clone as 66937-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66937-1-Ig (same clone as 66937-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66937-1-Ig (same clone as 66937-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66937-1-Ig (same clone as 66937-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66937-1-Ig (same clone as 66937-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66937-1-Ig (UBR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66937-1-Ig (same clone as 66937-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66937-1-Ig (UBR5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66937-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66937-1-PBS cible UBR5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UBR5 Protéine recombinante Ag18753
• Nom complet: ubiquitin protein ligase E3 component n-recognin 5
• Masse moléculaire calculée: 2799 aa, 309 kDa
• Poids moléculaire observé: 309 kDa
• Numéro d’acquisition GenBank: BC137234
• Symbole du gène: UBR5
• Identification du gène (NCBI): 51366
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UBR5, also named as ubiquitin protein ligase E3 component n-reconin5, is a HECT domain-containing protein as a myocardin-binding protein. UBR5 is highly conserved in metazoans, has unique structural features, and has been implicated in regulation of DNA damage response, metabolism, transcription, and apoptosis. Hence, UBR5 is a key regulator of cell signaling relevant to broad areas of cancer biology (PMID: 26464214). The human UBR5 gene has 59 exons, encoding an approximately 10kb mRNA and >300 kDa protein with widespread expression in various cell types.

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