Anticorps Polyclonal de lapin anti-UBXN1

• Phare
• Validé par KD/KO

UBXN1 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 16135-1-AP

Synonymes

2B28, LOC51035, SAKS1, SAPK substrate protein 1, UBA/UBX 33.3 kDa protein, UBX domain protein 1, UBXD10, UBXN1

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Galerie de données de validation

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  WB analysis using 16135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16135-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 16135-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16135-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16135-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16135-1-AP (UBXN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16135-1-AP (UBXN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Ramos, cellules SH-SY5Y, tissu cardiaque humain, tissu cérébral humain, tissu de cervelet de souris, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:30000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

16135-1-AP cible UBXN1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBXN1 Protéine recombinante Ag9146
• Nom complet: UBX domain protein 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC000902
• Symbole du gène: UBXN1
• Identification du gène (NCBI): 51035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	EMBO J; A weakened interface in the P182L variant of HSP27 associated with severe Charcot-Marie-Tooth neuropathy causes aberrant binding to interacting proteins.; Authors - T Reid Alderson
human	WB	Cancer Lett; The CRISPR/Cas9 system targeting EGFR exon 17 abrogates NF-κB activation via epigenetic modulation of UBXN1 in EGFRwt/vIII glioma cells.; Authors - Kai Huang
human,mouse	WB,IHC	Front Immunol; PTRF/Cavin-1 as a Novel RNA-Binding Protein Expedites the NF-κB/PD-L1 Axis by Stabilizing lncRNA NEAT1, Contributing to Tumorigenesis and Immune Evasion in Glioblastoma.; Authors - Kaikai Yi
human	WB	Sci Rep; PMID: 29769705; Authors - Shotaro Uchida
human	WB,IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli ; KO Validated
human	WB,IP,CoIP,IF	Mol Cell Biol; The VCP-UBXN1 complex mediates triage of ubiquitylated cytosolic proteins bound to the BAG6 complex.; Authors - Rakesh Ganji ; KD Validated