Anticorps Polyclonal de lapin anti-UBXD1

• Phare
• Validé par KD/KO

UBXD1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 14706-1-AP

Synonymes

UBX domain protein 6, UBXD1, UBXDC2, UBXN6

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Galerie de données de validation

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  WB analysis of PC-3 using 14706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14706-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14706-1-AP (UBXD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14706-1-AP (UBXD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14706-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14706-1-AP (UBXD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of Hela cells, using UBXN6 antibody 14706-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HeLa using 14706-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14706-1-AP (UBXD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris, tissu cérébral humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

14706-1-AP cible UBXD1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBXD1 Protéine recombinante Ag6394
• Nom complet: UBX domain protein 6
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC008288
• Symbole du gène: UBXN6
• Identification du gène (NCBI): 80700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase.; Authors - Xiaojun Wu ; KD Validated
human	IF	Acta Neuropathol Commun; Proximity proteomics of C9orf72 dipeptide repeat proteins identifies molecular chaperones as modifiers of poly-GA aggregation.; Authors - Feilin Liu
human	WB	J Immunol; UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling.; Authors - Harshada Ketkar
human	IF	J Cell Sci; The p97-UBXN1 complex regulates aggresome formation.; Authors - Sirisha Mukkavalli

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tingting (Verified Customer) (01-16-2020)

Very clear bands

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Trophoblast cell, HEK293T