Anticorps Monoclonal anti-UCH-L1/PGP9.5

• Phare
• Validé par KD/KO

UCH-L1/PGP9.5 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1C9E11

N° de cat : 66230-1-PBS

Synonymes

PGP9.5, UCHL1, UCHL1/PGP9.5, 1C9E11, EC:3.4.19.12

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Galerie de données de validation

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  WB analysis using 66230-1-Ig (same clone as 66230-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66230-1-Ig (same clone as 66230-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66230-1-Ig (UCHL1 Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66230-1-Ig (UCHL1/PGP9.5 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66230-1-Ig (same clone as 66230-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using UCH-L1/PGP9.5 antibody (66230-1-Ig, Clone: 1C9E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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  FC experiment of Y79 using 66230-1-Ig (same clone as 66230-1-PBS)

  1x10^6 Y79 cells were intracellularly stained with 0.4 ug UCHL1/PGP9.5 Monoclonal antibody (66230-1-Ig, Clone:1C9E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66230-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66230-1-PBS cible UCH-L1/PGP9.5 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UCH-L1/PGP9.5 Protéine recombinante Ag6547
• Nom complet: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC000332
• Symbole du gène: UCHL1
• Identification du gène (NCBI): 7345
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

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