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Anticorps Monoclonal anti-UCHL1/PGP9.5

UCHL1/PGP9.5 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1C9E11

N° de cat : CL594-66230

Synonymes

Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1, UCHL1/PGP9.5

Galerie de données de validation

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  • Flow cytometry (FC) experiment of Y79 cells using CoraLite®594-conjugated UCHL1/PGP9.5 Monoclonal an (CL594-66230)

    FC experiment of Y79 using CL594-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human UCHL1/PGP9.5 (CL594-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en cytométriecellules Y79,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66230 cible UCHL1/PGP9.5 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène UCHL1/PGP9.5 Protéine recombinante Ag6547
Nom complet ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Masse moléculaire calculée 25 kDa
Poids moléculaire observé 27 kDa
Numéro d’acquisition GenBankBC000332
Symbole du gène UCHL1
Identification du gène (NCBI) 7345
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

Protocole

Product Specific Protocols
FC protocol for CL594 UCHL1/PGP9.5 antibody CL594-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,ratFC (Intra)

Br J Pharmacol

Dopamine regulates colonic glial cell-derived neurotrophic factor secretion through cholinergic dependent and independent pathways

Authors - Xiao-Li Zhang
View Article