Anticorps Recombinant de lapin anti-UCHL3

UCHL3 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242015A10

N° de cat : 84542-6-PBS

Synonymes

UCH-L3, UCH L3, Ubiquitin thioesterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, EC:3.4.19.12

---

Galerie de données de validation

• 

  WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 84542-6-RR (same clone as 84542-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84542-6-RR (UCHL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 84542-6-RR (same clone as 84542-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84542-6-RR (UCHL3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84542-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84542-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84542-6-RR against Human UCHL3 were performed. The affinity constant is 2.45 nM.

---

Informations sur le produit

84542-6-PBS cible UCHL3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UCHL3 Protéine recombinante Ag3053
• Nom complet: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC018125
• Symbole du gène: UCHL3
• Identification du gène (NCBI): 7347
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.

{{ptg:RelatedPrimaryAntibodies}}