Anticorps Polyclonal de lapin anti-UCHL5

• Phare
• Validé par KD/KO

UCHL5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11527-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse colon using 11527-1-AP (same clone as 11527-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11527-1-AP (same clone as 11527-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11527-1-PBS cible UCHL5 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCHL5 Protéine recombinante Ag2095
• Nom complet: ubiquitin carboxyl-terminal hydrolase L5
• Masse moléculaire calculée: 326 aa, 37 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC025369
• Symbole du gène: UCHL5
• Identification du gène (NCBI): 51377
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).

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