Anticorps Polyclonal de lapin anti-UCK1

UCK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 12271-1-AP

Synonymes

Uridine-cytidine kinase 1, Uridine monophosphokinase 1, uridine cytidine kinase 1, UCK 1, EC:2.7.1.48

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Galerie de données de validation

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  WB analysis using 12271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12271-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12271-1-AP

  IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug.

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  IF Staining of MCF-7 using 12271-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12271-1-AP cible UCK1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCK1 Protéine recombinante Ag2912
• Nom complet: uridine-cytidine kinase 1
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC015547
• Symbole du gène: UCK1
• Identification du gène (NCBI): 83549
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Uridine/cytidine kinase-1 is a pyrimidine ribonucleoside kinase that catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP). The deduced 277-amino acid protein has a calculated molecular mass of 31 kDa. UCK1 belongs to the uridine kinase family and UCK have important roles for the phosphorylation of nucleoside analogs that may be important in chemotherapy of cancer.

Publications

Species	Application	Title
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai
human,mouse	WB	Blood Adv; Reprogramming of pyrimidine nucleotide metabolism supports vigorous cell proliferation of normal and malignant T cells; Authors - Tatsuro Watanabe
human	WB	Nat Metab; Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition; Authors - Minwoo Nam
human	WB	Oncogene; The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation; Authors - Nannan Sha
		Mol Cancer Res; EIF4A3 acts on the PI3K-AKT-ERK1/2-P70S6K pathway through FLOT1 to influence the development of lung adenocarcinoma; Authors - Wenhao Yu
human	WB,IP,IF	Cell Rep; mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase; Authors - Brittany Q Pham

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody is good considering the low expression of UCK1 protein. I could see a thick band that corresponds with UCK1 MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

UCK1 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:12271-1-AP)

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