Anticorps Polyclonal de lapin anti-UCKL1

UCKL1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17005-1-AP

Synonymes

F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

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Galerie de données de validation

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  WB analysis of K-562 using 17005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 17005-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 17005-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 17005-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Raji, cellules U-937, tissu rénal de rat
• Résultats positifs en IHC: tissu rénal de souris, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

17005-1-AP cible UCKL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCKL1 Protéine recombinante Ag10701
• Nom complet: uridine-cytidine kinase 1-like 1
• Masse moléculaire calculée: 419 aa, 46 kDa
• Poids moléculaire observé: 61 kDa, 46 kDa
• Numéro d’acquisition GenBank: BC033078
• Symbole du gène: UCKL1
• Identification du gène (NCBI): 54963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	EBioMedicine; Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer; Authors - Weili Wu ; KD Validated