Anticorps Polyclonal de lapin anti-UCKL1

UCKL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28762-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28762-1-AP (same clone as 28762-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28762-1-AP (UCKL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 28762-1-AP (same clone as 28762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 28762-1-AP (same clone as 28762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28762-1-PBS cible UCKL1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCKL1 Protéine recombinante Ag30535
• Nom complet: uridine-cytidine kinase 1-like 1
• Masse moléculaire calculée: 419 aa, 46 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC033078
• Symbole du gène: UCKL1
• Identification du gène (NCBI): 54963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Uridine cytidine kinase like-1 (UCKL-1) is a largely uncharacterized protein over-expressed in many tumor cells, especially in highly malignant, aggressive tumors. Sequence analysis indicates that UCKL-1 has homology to uridine kinases, enzymes that play a role in DNA and RNA synthesis and that are often up-regulated in tumor cells. Previous studies have shown that UCKL-1 is a substrate for natural killer lytic-associated molecule (NKLAM), an E3 ubiquitin ligase found in NK cell cytolytic granules. Ubiquitination of UCKL-1 by NKLAM leads to its degradation. Increased expression of NKLAM enhances NK-mediated tumoricidal activity. (PMID: 32083188)

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