Anticorps Polyclonal de lapin anti-Urocortin

Urocortin Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 19731-1-AP

Synonymes

UCN, UI, UROC

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Galerie de données de validation

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  WB analysis using 19731-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19731-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19731-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 19731-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19731-1-AP (Urocortin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 19731-1-AP

  IP result of anti-Urocortin (IP:19731-1-AP, 4ug; Detection:19731-1-AP 1:1000) with mouse kidney tissue lysate 2160 ug.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, tissu rénal humain, tissu testiculaire humain
• Résultats positifs en IP: tissu rénal de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

19731-1-AP cible Urocortin dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: urocortin
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_003353
• Symbole du gène: UCN
• Identification du gène (NCBI): 7349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).

Publications

Species	Application	Title
human	WB,IHC	J Cancer Res Clin Oncol; Urocortin-1 promotes colorectal cancer cell migration and proliferation and inhibits apoptosis via inhibition of the p53 signaling pathway; Authors - Xiaolan Guo

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