Anticorps Polyclonal de lapin anti-UCP1

UCP1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

79

Avis (1)

N° de cat : 23673-1-AP

Synonymes

SLC25A7, Thermogenin, UCP, UCP 1, UCP1

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Galerie de données de validation

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  WB analysis using 23673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23673-1-AP (UCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brown adipose using 23673-1-AP

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 23673-1-AP (UCP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 23673-1-AP (UCP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat brain using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 23673-1-AP (UCP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brown adipose using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 23673-1-AP (UCP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 23673-1-AP (UCP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu adipeux brun de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu adipeux brun de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 68 publications below
• IHC: See 23 publications below
• IF: See 7 publications below

Informations sur le produit

23673-1-AP cible UCP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCP1 Protéine recombinante Ag20388
• Nom complet: uncoupling protein 1 (mitochondrial, proton carrier)
• Masse moléculaire calculée: 307 aa, 33 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC098168
• Symbole du gène: UCP1
• Identification du gène (NCBI): 7350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Publications

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu ; KO Validated
mouse	WB	Nat Commun; Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages.; Authors - Yawei Wang
mouse	WB,IHC	Mol Biol Evol; Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans; Authors - Ming Zhou
mouse	WB	Diabetes; Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.; Authors - Hui Zhao
mouse	WB	JCI Insight; Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism; Authors - Benedict Abdon
	WB	Diabetes; PMID: 23990362; Authors - Wang Qun Q

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (03-06-2023)

It works well in human brown adipocyte cells. Although we got several non-specific bands, the band at the expected size was very clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human brown adipocytes

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