Anticorps Monoclonal anti-UCP2

UCP2 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B4B1

N° de cat : 66700-1-PBS

Synonymes

2B4B1, BMIQ4, Dicarboxylate carrier SLC25A8, SLC25A8, UCP 2

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Galerie de données de validation

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  WB analysis of Jurkat using 66700-1-Ig (same clone as 66700-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

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  WB analysis of human adipose using 66700-1-Ig (same clone as 66700-1-PBS)

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66700-1-Ig (same clone as 66700-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66700-1-PBS cible UCP2 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UCP2 Protéine recombinante Ag19361
• Nom complet: uncoupling protein 2 (mitochondrial, proton carrier)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC011737
• Symbole du gène: UCP2
• Identification du gène (NCBI): 7351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.

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