Anticorps Polyclonal de lapin anti-UCP3

UCP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 10750-1-AP

Synonymes

SLC25A9, UCP 3, UCP3

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Galerie de données de validation

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  WB analysis using 10750-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10750-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 10750-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10750-1-AP (UCP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10750-1-AP (UCP3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10750-1-AP (UCP3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10750-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10750-1-AP (UCP3 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules SGC-7901, tissu de muscle squelettique de rat
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 1 publications below

Informations sur le produit

10750-1-AP cible UCP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UCP3 Protéine recombinante Ag1134
• Nom complet: uncoupling protein 3 (mitochondrial, proton carrier)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC008392
• Symbole du gène: UCP3
• Identification du gène (NCBI): 7352
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; DNA Damage Promotes TMPRSS2-ERG Oncoprotein Destruction and Prostate Cancer Suppression via Signaling Converged by GSK3β and WEE1.; Authors - Zhe Hong
mouse	WB	Circ Res; Uncoupling protein 2 deficiency mimics the effects of hypoxia and endoplasmic reticulum stress on mitochondria and triggers pseudohypoxic pulmonary vascular remodeling and pulmonary hypertension.; Authors - Dromparis Peter P
mouse	WB	EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat	WB,IHC	Cell Physiol Biochem; PMID: 29669326; Authors - Yintao Chen
mouse	WB	J Mol Cell Cardiol; Impaired SIRT3 activity mediates cardiac dysfunction in endotoxemia by calpain-dependent disruption of ATP synthesis.; Authors - Christoph Koentges
mouse	WB	Sci Rep; Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis.; Authors - María Rodríguez-Hidalgo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edma (Verified Customer) (03-03-2025)

I think the antibody was not that specific for our target in A549 cells, and the western blot didn't work. we got a lot of unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells

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