Anticorps Polyclonal de lapin anti-UFD1L

• Phare
• Validé par KD/KO

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(20)

Avis (1)

N° de cat : 10615-1-AP

Synonymes

UB fusion protein 1, Ubiquitin fusion degradation protein 1, Ubiquitin recognition factor in ER-associated degradation protein 1, UFD1

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Galerie de données de validation

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  WB analysis of K-562 using 10615-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10615-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 10615-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10615-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10615-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 10615-1-AP

  IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.

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  IHC staining of human stomach using 10615-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 10615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10615-1-AP

  Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10615-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2, cellules Raji, tissu de thymus de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu d'estomac humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa
• Résultats positifs en FC (Intra): cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10615-1-AP cible UFD1L dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UFD1L Protéine recombinante Ag0954
• Nom complet: ubiquitin fusion degradation 1 like (yeast)
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC005087
• Symbole du gène: UFD1L
• Identification du gène (NCBI): 7353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
human	WB	Nature; ESCRT-III controls nuclear envelope reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Elife; The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP; Authors - Maria Körner
mouse	WB	Sci Signal; TRIM55 promotes noncanonical NF-κB signaling and B cell-mediated immune responses by coordinating p100 ubiquitination and processing; Authors - Liangbin Lin
	WB	Plant J; The ubiquitin-26S proteasome system and autophagy relay proteome homeostasis regulation during silique development.; Authors - Peifeng Yu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Leslie (Verified Customer) (10-08-2019)

Excellent antibody for Western blots

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Skeletal Muscle

UFD1L Antibody Western Blot, validation (1:1000 dilution) in Skeletal Muscle (Cat no:10615-1-AP)

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