Anticorps Polyclonal de lapin anti-UGDH

• Phare
• Validé par KD/KO

UGDH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 13151-1-AP

Synonymes

UDP-glucose 6-dehydrogenase, UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH

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Galerie de données de validation

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  WB analysis using 13151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13151-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13151-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 13151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using UGDH antibody (13151-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Informations sur le produit

13151-1-AP cible UGDH dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UGDH Protéine recombinante Ag3767
• Nom complet: UDP-glucose dehydrogenase
• Masse moléculaire calculée: 494 aa, 55 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC022781
• Symbole du gène: UGDH
• Identification du gène (NCBI): 7358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Publications

Species	Application	Title
human	WB	Nature; UDP-glucose accelerates SNAI1 mRNA decay and impairs lung cancer metastasis.; Authors - Xiongjun Wang ; KD Validated
human	WB, IHC	Arthritis Res Ther; UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis.; Authors - Yinxian Wen ; KD Validated
human	WB	PLoS One; Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.; Authors - Qi Ying
human	WB, IHC	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human,mouse	WB	Nat Commun; UDP-glucuronate metabolism controls RIPK1-driven liver damage in nonalcoholic steatohepatitis; Authors - Tao Zhang
human	WB,IHC	Arch Biochem Biophys; UGDH promotes 5-fluorouracil resistance in colorectal cancer via the ROS-activated PI3K/AKT-EEF1A2-PRDX1 pathway; Authors - Jinfeng Cai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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