Anticorps Monoclonal anti-UGDH

• Phare
• Validé par KD/KO

UGDH Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E2E1

N° de cat : 67360-1-PBS

Synonymes

UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH, EC:1.1.1.22

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Galerie de données de validation

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  WB analysis using 67360-1-Ig (same clone as 67360-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 67360-1-Ig (same clone as 67360-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67360-1-Ig (same clone as 67360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67360-1-Ig (same clone as 67360-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67360-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67360-1-PBS cible UGDH dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: UGDH Protéine recombinante Ag29747
• Nom complet: UDP-glucose dehydrogenase
• Masse moléculaire calculée: 494 aa, 55 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC022781
• Symbole du gène: UGDH
• Identification du gène (NCBI): 7358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

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