Anticorps Polyclonal de lapin anti-UGT8

• Phare
• Validé par KD/KO

UGT8 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 17982-1-AP

Synonymes

ceramide galactosyltransferase, Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8

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Galerie de données de validation

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  WB analysis of rat brain using 17982-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17982-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 17982-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 17982-1-AP

  IP Result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 17982-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

17982-1-AP cible UGT8 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UGT8 Protéine recombinante Ag12393
• Nom complet: UDP glycosyltransferase 8
• Masse moléculaire calculée: 541 aa, 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC075069
• Symbole du gène: UGT8
• Identification du gène (NCBI): 7368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.

Publications

Species	Application	Title
human	WB,IF	J Exp Med; Inhibition of UGT8 suppresses basal-like breast cancer progression by attenuating sulfatide-αVβ5 axis.; Authors - Qianhua Cao ; KD Validated
mouse,human	WB,IHC	Mol Cell Proteomics; Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis.; Authors - Michael R Heaven
human	WB	Mol Pharmacol; Circular RNAs of UDP-Glycosyltransferase (UGT) Genes Expand the Complexity and Diversity of the UGT Transcriptome.; Authors - Dong Gui Hu
human	IHC	Biosci Rep; Uronic acid metabolic process-related gene expression-based signature predicts overall survival of glioma.; Authors - Yuemei Feng
rat, human	WB,IHC	Cancer Chemother Pharmacol; Evaluation of resveratrol sensitivities and metabolic patterns in human and rat glioblastoma cells.; Authors - Sun Zheng Z
rat	WB, IHC	Neurotherapeutics; Diffusion Efficiency and Bioavailability of Resveratrol Administered to Rat Brain by Different Routes: Therapeutic Implications.; Authors - Xiao-Hong Shu