Anticorps Recombinant de lapin anti-UHRF1

• Phare
• Validé par KD/KO

UHRF1 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241872B12

N° de cat : 84489-4-PBS

Synonymes

241872B12, E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1

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Galerie de données de validation

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  WB analysis using 84489-4-RR (same clone as 84489-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84489-4-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 84489-4-RR (same clone as 84489-4-PBS)

  WB result of UHRF1 antibody (84489-4-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UHRF1 transfected HEK-293T cells. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84489-4-RR (same clone as 84489-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-4-RR, Clone: 241872B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84489-4-RR (same clone as 84489-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug UHRF1 Recombinant antibody (84489-4-RR, Clone:241872B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84489-4-PBS in a different storage buffer formulation.

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Informations sur le produit

84489-4-PBS cible UHRF1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: UHRF1 Protéine recombinante Ag17498
• Nom complet: ubiquitin-like with PHD and ring finger domains 1
• Masse moléculaire calculée: 806 aa, 91 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC113875
• Symbole du gène: UHRF1
• Identification du gène (NCBI): 29128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

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