Anticorps Recombinant de lapin anti-ULBP2

• Phare
• Validé par KD/KO

ULBP2 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

240413A6

N° de cat : 83709-1-PBS

Synonymes

240413A6, ALCAN alpha, ALCAN-alpha, N2DL2, N2DL-2

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Galerie de données de validation

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  WB analysis using 83709-1-RR (same clone as 83709-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83709-1-RR (ULBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 83709-1-RR (same clone as 83709-1-PBS)

  WB result of ULBP2 antibody (83709-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ULBP2 transfected Jurkat cells. This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83709-1-RR (same clone as 83709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 83709-1-RR (same clone as 83709-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83709-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83709-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83709-1-RR against Human ULBP2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83709-1-PBS cible ULBP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ULBP2 Protéine recombinante Eg0858
• Nom complet: UL16 binding protein 2
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_025217.4
• Symbole du gène: ULBP2
• Identification du gène (NCBI): 80328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

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