Anticorps Polyclonal de lapin anti-ULK1

ULK1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 29005-1-AP

Synonymes

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 29005-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 29005-1-AP (ULK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 29005-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (29005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

29005-1-AP cible ULK1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-51-like kinase 1 (C. elegans)
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: NM_003565
• Symbole du gène: ULK1
• Identification du gène (NCBI): 8408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Publications

Species	Application	Title
goat	WB	Ecotoxicol Environ Saf; Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells; Authors - Shuaiqi Han
human	WB	Sci Rep; EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagy; Authors - Zhen Xu
human	WB	Cytojournal; Exosomes derived from cancer-associated fibrolasts mediated ciplatin resistance; Authors - Yiyu Meng
mouse	WB	Sci China Life Sci; GLA deficiency causes cardiac hypertrophy via enhanced autophagy; Authors - Wei Ou
mouse	WB,IHC,IF	Aging Cell; DLK/JNK3 Upregulation Aggravates Hair Cell Senescence in Mice Cochleae via Excessive Autophagy; Authors - Rui Ding
human		Med Oncol; GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells; Authors - Weiting He

{{ptg:RelatedPrimaryAntibodies}}